Journal: Oncology Reports

Article Title: Multidrug resistance protein 5 affects cell proliferation, migration and gemcitabine sensitivity in pancreatic cancer MIA Paca‑2 and PANC‑1 cells

doi: 10.3892/or.2023.8666

Figure Lengend Snippet: Representative Transwell migration images of (A) MIA Paca-2 and (B) PANC-1 clones transduced with scrambled control and multidrug resistance protein 5-short-hairpin RNA (scale bars, 200 µm). Migrated cells were quantified as a mean percentage of scrambled control in (C) MIA Paca-2 and (D) PANC-1 cells. The bars represent the mean and standard deviation from three independent experiments performed in duplicates. ***P<0.001; ****P<0.0001 from Dunnett's post-hoc test that followed one-way ANOVA.

Article Snippet: Cells in serum-free DMEM were seeded in the Corning ® Costar ® Transwell ® cell culture inserts (cat. no. CLS3464; Sigma-Aldrich; Merck KGaA) at a density of 3×10 5 cells per insert.

Techniques: Migration, Clone Assay, Transduction, shRNA, Standard Deviation

Journal: Science Advances

Article Title: Ultrathin micromolded 3D scaffolds for high-density photoreceptor layer reconstruction

doi: 10.1126/sciadv.abf0344

Figure Lengend Snippet: ( A to C ) Low-magnification photographic images depicting scaffold mounting into the transwell insert. (A) Transwell insert with PGS scaffold below. The outer edge of the scaffold was glued to the transwell insert with soft PDMS. The area of the transwell insert removed to mount scaffolds was 19.6 mm 2 (internal diameter, 5 mm). (B) Transwell insert holder with a PGS ice cube tray scaffold mounted into a transwell insert. (C) Six-transwell scaffold cell culture system. ( D to F ) Laminin-coated ice cube tray scaffolds are readily filled with hPSC-derived CRX +/tdTomato -expressing PRs. (D) 3D rendering of a scaffold (176 μm by 185 μm by 22 μm) confirms successful capture of multiple PRs (labeled in red) in individual capture wells. Cell nuclei are labeled with 4′,6-diamidino-2-phenylindole (DAPI) (blue). (E) Cells were seeded onto scaffolds at varying densities to determine the minimum number required to achieve the maximum carrying capacity of CRX +/tdTomato -PRs per well. Median (bold dashes) and quartiles (fine dashes) are shown within individual violin plots. (F) Scaffolds seeded with CRX +/tdTomato -PRs (RFP + , red) contain both ARR3-expressing cone PRs (green) and NR2E3-expressing rod PRs (pink). A 3D lateral view of the scaffold demonstrates relatively even distribution of ARR3 + cones and NR2E3 + rods. 3D rendering is 644 μm by 644 μm by 20 μm. Photo Credit: In-Kyu Lee, Department of Electrical and Computer Engineering, University of Wisconsin–Madison.

Article Snippet: To facilitate cell seeding, we incorporated scaffolds into a commercially available 12-mm polyester transwell cell culture insert (Corning Costar Snapwell, Sigma-Aldrich) before sterilization ( ).

Techniques: Cell Culture, Derivative Assay, Expressing, Labeling